Permeability Barrier: Methods and Protocols - Methods in Molecular Biology 2011 edition

Marc Notes: Includes bibliographical references and index. Table of Contents:1. Computational Modeling of the Skin Barrier Arne Naegel, Michael Heisig, and Gabriel Wittum 2. In Vitro Human Skin Segmentation and Drug Concentration-Skin Depth Profiles Ana Melero, Tsambika Hahn, Ulrich F. Schaefer, and Marc Schneider 3. Transcriptional Regulation of Epidermal Barrier Formation Ambica Bhandari, Michael L. Salmans, William Gordon, and Bogi Andersen 4. Epidermal Permeability Barrier (EPB) Measurement in Mammalian Skin Arup Kumar Indra and Mark Leid 5. Assessment of Permeability Barriers to Macromolecules in the Rodent Endometrium at the Onset of Implantation Brent M. Bany and G. Scot Hamilton 6. Assessment of Intestinal Permeability in (Premature) Neonates by Sugar Absorption Tests Willemijn E. Corpeleijn, Ruurd M. van Elburg, Ido P. Kema, and Johannes B. van Goudoever 7. Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium Carrie A. Duckworth and Alastair J. Watson 8. Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells Rino P Donato, Adaweyah El-Merhibi, Batjargal Gundsambuu, Kai Yan Mak, Emma R Formosa, Xian Wang, Catherine A Abbott, and Barry C Powell 9. Optimization of the Caco-2 Permeability Assay to Screen Drug Compounds for Intestinal Absorption and Efflux Barry Press 10. Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion Isabel Larre, Ruben G. Contreras, and Marcelino Cereijido 11. Monitoring of the Dynamics of Epithelial Dome FormationUsing a Novel Culture Chamber for Long-Term Continuous Live-Cell Imaging Judith Lechner, Daniel Hekl, Helmut Gatt, Markus Voelp, and Thomas Seppi 12. Measuring Permeability in Human Retinal Epithelial Cells (ARPE-19): Implications for the Study of Diabetic Retinopathy Marta Garcia-Ramirez, Marta Villarroel, Lidia Corraliza, Cristina Hernandez, and Rafael Simo 13. Analysis of Epithelial Barrier Integrity in Polarized Lung Epithelial Cells Monika Strengert and Ulla G. Knaus 14. Permeability of Differentiated Human Urothelium In Vitro Peter Rubenwolf and Jennifer Southgate 15. Phenotyping the Claudin 11 Deficiency in Testis: From Histology to Immunohistochemistry Severine Mazaud-Guittot, Alexander Gow, and Brigitte Le Magueresse-Battistoni 16. An In Vitro System to Study Sertoli Cell Blood-Testis Barrier Dynamics Dolores D. Mruk and C. Yan Cheng 17. Analysis of Endothelial Barrier Function In Vitro Yuping Wang and J. Steven Alexander 18. Role of Endothelial Cell-Cell Junctions in Endothelial Permeability Armelle Le Guelte and Julie Gavard 19. In Vitro Analyses of Endothelial Cell Permeability Elizabeth Monaghan-Benson and Erika S. Wittchen 20. Mechano-Transduction and Barrier Regulation in Lung Microvascular Endothelial Cells Kristina Giantsos, Mark Cluff, and Randal Dull 21. Role of Caveolin-1 in the Regulation of Pulmonary Endothelial Permeability Yu Sun, Richard D. Minshall, and Guochang Hu 22. Assessment of Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cell Monolayers Andreas Ludwig, Anselm Sommer, and Stefan Uhlig 23. Permeability of Endothelial Barrier: Cell Culture and In Vivo Models Alexander N. Garcia, Stephen M. Vogel, Yulia A. Komarova, and Asrar B. Malik 24. Size-Selective and In Vitro Assessment of Inner Blood Retina Barrier Permeability Matthew Campbell and Peter Humphries 25. Assessment of Permeability in Barrier Type of Endothelium in Brain Using Tracers: Evans Blue, Sodium Fluorescein, and Horseradish Peroxidase Mehmet Kaya and Bulent Ahishali 26. In Vitro and In Vivo Methods for Assessing FcRn-Mediated Reverse Transcytosis Across the Blood-Brain Barrier Nadia Caram-Salas, Eve Boileau, Graham Farrington, Ellen Garber, Eric Brunette, Abedelnasser Abulrob, and Danica Stanimirovic 27. Evaluation of VEGF-Induced Vascular Permeability in Mice Sara M. Weis 28. In Vivo Measurement of Glioma-Induced Vascular Permeability Jisook Lee, Andrew Baird, and Brian P. Eliceiri 29. In Vivo Optical Imaging of Ischemic Blood Brain Barrier Disruption Abedelnasser Abulrob, Eric Brunette, Jacqueline Slinn, Ewa Baumann, and Danica Stanimirovic"Jacket Description/Back: The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body. In "Permeability Barrier: Methods and Protocols," a variety of experienced researchers contribute techniques to study this complex system in its many forms. Written in the highly successful "Methods in Molecular Biology " series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, "Permeability Barrier: Methods and Protocols" serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research."